LGC offers proprietary and DNA extraction solutions to deliver high quality DNA preparations. LGC DNA extraction kits include a range of different extraction technologies and reflect the capacity to develop tailor-made kit systems optimized to any particular application.
LGC reliable robotic equipment stands for high throughput in combination with high quality DNA extraction solutions, perfectly adapted to the proprietary chemistry.
The genomics services include high quality DNA and RNA extraction solutions customized to any application using specification of your project
Sample material can vary greatly in consistency as well as in sugar, protein and fat composition - all of which can affect the quality and purity of the extracted nucleic acid and the subsequent performance of downstream applications. To provide the most efficient and effective extraction method LGC offers not only a range of different extraction technologies but also the capacity to develop tailor-made kit systems optimized to a particular application.
The table below describes a number of the key features of each of LGC extraction kit technologies.
| *1 Our surface-coated superparamagnetic particles do not require the use of vacuum filtration or centrifugation steps, hence they are well-suited to automation. | ||||||
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sbeadex™ |
mag™ |
Kleargene silica |
Kleargene spin |
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Matrix |
Surface-modified superparamagnetic particles |
Modified magnetic particles |
Silica particles |
Glass fibre solid support |
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Mechanism of action*1 |
Novel two-step binding mechanism |
Classic polarity-based binding mechanism |
Classic polarity-based binding mechanism; |
Classic polarity-based binding mechanism; |
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Format |
96-well |
96-well |
single tubes |
96 and 384-well |
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Flexibility |
scalable protocols |
scalable protocols |
low to high volume extractions |
spin columns |
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Optimized matrices |
Plant material, forensic samples, tissue and cells, bacterial cultures |
Whole blood, buffy coat, buccal swabs, bacteria, yeast, fungi |
Blood, tissue and saliva samples |
Plant tissue typically 100ng to 10µg |
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Grade of automation |
Automated high-throughput extraction |
Automated high-throughput extraction |
Manual extraction |
Semi-automated high throughput extraction |
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Benefits |
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Significant advances in plant breeding have been facilitated by the rapid and continued advancement of molecular biology-based technologies. These are driving the need for new automated extraction platforms that combine higher sample throughputs with the delivery of highly purified DNA that is suitable for a wide range of downstream applications.
The oKtopure™ robot has been developed to deliver automated DNA extraction from 8 x 96 well microtitre plates in parallel; utilizing the proprietary sbeadex magnetic bead based chemistry. The instrument delivers standardized, medium-throughput extractions and results in purification of high quality DNA suitable for all molecular biology based downstream applications including sequencing (NGS, Sanger) and SNP genotyping (e.g. KASP™).
The Genespin™ instrument delivers a higher sample throughput and is optimized for Kleargene, LGC spin plate-based DNA extraction chemistry. The instrument delivers standardized, semi-automated DNA extractions for downstream PCR-based applications. The instrument is compatible with both 96- and 384-well spin plates, and can be used to perform all lysate transfer and buffer addition steps.
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Key feature |
oKtopure |
Genespin |
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Compatible chemistry |
sbeadex™ |
Kleargene |
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Extraction technology |
Surface-modified superparamagnetic particles |
Spin plates: glass fiber solid support inserted to microtitre plate |
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Plate format |
96-well |
96-well or 384-well |
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Grade of automation |
Fully automated |
Semi automated |
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Throughput |
up to 3.000 - 4.000 samples / day |
up to 15.000 - 20.000 samples / day |
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DNA quality |
Very high |
Very good |
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Consumables costs |
Low |
Very low |
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Sample type |
Leaf and seed tissue |
Leaf and seed tissue |
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Downstream applications |
All genomics based applications including Next-generation sequencing, Sanger sequencing, SNP genotyping |
PCR based applications |
The first step in any study investigating genetic differences in an organism of interest is the purification of DNA and the nucleic acid extraction teams at LGC provides DNA and RNA extraction services to meet the demands of any project.
Depending on project size, specific customer requirements and the biological background of the project, LGC can implement an extraction service solution using any one or combination of LGC different extraction technologies to provide and optimized fit-for-purpose solution. The range of genomics services includes high quality DNA and RNA extraction solutions.
LGC have developed extraction protocols from a wide range of starting materials and volumes which enable the delivery of off-the-shelf services for most applications or tailor-made solutions utilizing LGC unique range of in-house technologies.
LGC RNA extraction services include the ability to prepare RNA from:
- RNAlater® preserved material
- PAXgene® samples
- Frozen sample material.
LGC extraction laboratories are able to run a range of quality checks and DNA quality assessment procedures including:
- Quantification of DNA / RNA
- Concentration normalization of DNA extracts
- SNP DNA quality control
- Quality check of RNA with Agilent 2100 Bioanalyzer.
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