As a provider of genotyping products and services for over 10 years, LGC takes great pride in helping customers everyday to obtain high quality genotyping results that advance their research goals.
The KASP™ genotyping chemistry delivers accurate, flexible and cost-effective genotyping service solutions. Using our PCR-based KASP genotyping assay, in conjunction, with KASP Master mix, LGC is able to provide complete genotyping services to our customers. We run the largest genotyping laboratory of its kind in the world. We have a track record of delivering the highest quality data, delivering break-through cost savings across a huge range of project types, and working with hundreds of different species.
Genotyping and all-inclusive services
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Service |
Express Genotyping |
Regular Genotyping |
All-inclusive services |
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Prices |
Starting at <£0.15 per datapoint |
Starting at <£0.10 per datapoint |
Starting at £0.95 per sample |
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Turnaround time (reference) |
2 weeks |
4 weeks |
6 weeks |
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KASP™ is LGC proprietary genotyping technology.
The KASP genotyping reaction comprises three components:
The KASP Assay mix is specific to the SNP or InDel to be targeted and consists of two competitive, allele-specific forward primers and one common reverse primer. Each forward primer incorporates an additional tail sequence that corresponds with one of two universal FRET (fluorescent resonance energy transfer) cassettes present in the KASP Master mix. All custom KASP assays are available with the additional assay validation service.
KASP Master mix contains the two universal FRET cassettes (FAM and HEX), ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimized buffer solution.
- Sample DNA with the sequence of interest
- KASP Assay mix (containing the target specific primers)
- KASP Master mix.
KASP Master mix and KASP Assay mix are used in-house for LGC genotyping service projects but can also be purchased to run KASP genotyping reactions in your own laboratory.
SNPline workflow
A SNPline enables the user to generate anywhere from 1536 data points to 1,536,000 data points per day, and retain the flexibility to run individual repeats without consuming expensive arrays / chips and producing far more data than you need.
A flexible and highly scalable solution for PCR-based genotyping.
Developed and optimized over many years within our high-throughput genotyping service laboratories in Europe and North America, the SNPline™ workflow consists of all instruments, software, and validated procedures we use to establish a highly cost-effective, flexible, and scalable PCR-based genotyping solution. With an open-format design and modular framework, customers have the option of adopting one of two standard configurations: the SNPline Lite and SNPline XL, or they can build a custom configuration that incorporates equipment they already own.
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