Flownamics® has developed the FISP®, which is a sampling probe capable of withdrawing sterile, cell-free samples from fermentors and bioreactors. FISP® allows direct on-line sample transfer to a variety of analyzers, such as biochemistry and HPLC systems, as well as collection for off-line analysis. FISP® is a small, tube-shaped, sterilizable 316 stainless steel carrier which is surrounded by a tubular, micro-porous membrane.They are available for 12, 19 and 25 mm ports for use in laboratory, pilot or industrial scale vessels. FISP® has been and continues to be widely utilized in the fermentation and cell culture fields since it’s introduction to the market in 1996.
Withdraws Sterile, Cell-Free Samples from Fermenters, Bioreactors or Other Vessel Types
The FISP is inserted into a side or top port of the vessel. It is immersed into the fermentation broth or culture media and sterilized in-situ. A peristaltic pump (or the vessel’s own hydrostatic pressure) withdraws a sample through the tubular membrane and into the probe’s stainless steel network of grooves. The sample flows through the network of grooves into a central channel where the cell-free sample can be directed to an on-line analyzer, fraction collector, or sample container. Fluid flow rate is dependent on the viscosity of the media.
- Biochemistry analyzers
- Fraction collectors, Autosamplers
- Flow Injection Analysis (FIA) systems
- HPLC systems
- Biosensors
- Methanol analyzers
- Sterile, cell-free sampling for on-line or off-line analysis
- Autoclave, SIP, & CIP compatible
- Risk free sampling with 0.2 micron filter (sterile barrier)
- Fits small laboratory to large full-scale production vessels
- Collect and store samples for off-line analysis
- Directly interfaces the fermentor or bioreactor with:
- Minimal dead volume for consistent and accurate sampling, which reduces flush time
- Withstands the temperatures, pressures, viscosities, shear forces, and chemicals typical of fermentation environments
- Use with aerobic or anaerobic bacteria, yeast, fungi, algae, insect, and mammalian cell cultures
- Easy to use and maintain; no operator training required
- Can be used for submerged feeding/sterile media addition
- Interfaces with Flownamics Seg-Flow® Sampling System (additional information available)
Available For 25mm Ports: Standard & Safety Fits Into the Fermenter / Bioreactor Side Port Dead Volume: Approximately 0.24 to 0.44ml Immersion Depths: 90 & 115mm
- Available For 25mm Ports: Standard & Safety
- Fits Into the Fermenter / Bioreactor Side Port
- Dead Volume: Approximately 0.24 to 0.44ml
- Immersion Depths: 90 & 115mm
Available For 12 & 19mm Ports Fits Into the Fermenter / Bioreactor Top Port Dead Volume: Approximately 0.24 to 0.44ml Immersion Depths: 120, 200, 310, & 410mm
- Used with disposable SUB's
- FISP Model P-F12-200-02
- Available For 12 & 19mm Ports
- Fits Into the Fermenter / Bioreactor Top Port
- Dead Volume: Approximately 0.24 to 0.44ml
- Immersion Depths: 120, 200, 310, & 410mm
- Compatible with a kleenpak™ connectors from Pall Corp.
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