Protein A resins from the leading manufacturer of Protein A ligands. Repligen Protein A affinity resins help you optimize process economics while maintaining industry-standard performance.  |
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CaptivA® Protein A Affinity ResinThe value choice in Protein A resins. CaptivA® Protein A Affinity Resin is the first agarose-based resin that delivers excellent binding capacity, caustic stability and low leaching at a fraction of the cost of traditional resins. Technology  CaptivA® Protein A Affinity Resin combines an industry standard cross-linked agarose bead with recombinant native Staphylococcal Protein A (rSPA) manufactured by Repligen, the world’s leading manufacturer of recombinant Protein A.
Base Matrix
Particle Size Ligand
Benefits 
Performance 
Dynamic Binding Capacity
Protein A Leaching
Caustic Stability
Recommended Flow Velocity
Recommended pH and Temperature Stability High dynamic binding capacity CaptivA® Protein A Affinity Resin delivers high dynamic binding capacity at standard process flow rates and residence times.
Sanitization stability CaptivA® Protein A Affinity Resin retains desired dynamic binding capacity when exposed to stringent washing conditions that reduce standard feed stream contaminants. The CaptivA® recombinant Protein A attachment mechanism creates multiple covalent attachment points between the ligand and agarose bead resulting in superior alkali resistance compared to single point attachment chemistries.
The human polyclonal IgG dynamic binding capacity was determined following 0.1 N NaOH exposure. A 5 x 50mm column was loaded with a 2 mg/ml hIgG solution in PBS pH 7.4. The loading residence time was 3 minutes. Capacity was determined and reported at 2% breakthrough. After elution, the column was exposed to 0.1 N NaOH for 5 hours. Five cycles were completed. An exposure time of 25 hours represents 100 x 15 minute CIP cycles. Low Protein A leaching CaptivA® Protein A Affinity Resin multi-point protein chemistry consistently delivers low Protein A leaching.
Protein A leaching from CaptivA® Protein A Resin is consistently OPUS® Columns pre-packed with CaptivA® Affinity Resin 
CaptivA® Protein A Affinity Resin is available pre-packed into OPUS® chromatography columns, combining the superior process economics of CaptivA® resin with OPUS® Column ready-to-use convenience and flexible column size options.
Economics CaptivA® Protein A Affinity Resin is the most cost-effective chromatography resin available today, delivering industry standard performance at a fraction of the cost of traditional affinity resins, especially when pre-packed into OPUS® chromatography columns.
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Dynamic binding capacity was determined for human polyclonal IgG at four different flow velocities providing residence times ranging from 0.5 to 6 minutes. A 5 x 50 mm column was loaded with a 2 mg/ml hIgG solution in PBS pH 7.4. Capacity was determined and reported at 2, 5, and 10% breakthrough. A column bed height between 10-20 cm, loaded at 200 cm/hr provides 6-12 minutes of residence time.
The human polyclonal IgG binding capacity was determined following each column cycle. Cleaning was performed between cycles with either extra volumes of Glycine pH 3.0 elution buffer or 0.1 N NaOH with a 15 minute contact time. Leached Protein A was measured in the product elution pool from each cycle using the Repligen Protein A ELISA Kit.
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